IDENTIFICAÇÃO DE POSSÍVEIS ESTs DE Eucalyptus RELACIONADOS A GENES RESPONSÁVEIS PELO ALONGAMENTO CELULAR
DOI:
https://doi.org/10.24278/2178-5031.2012242393Palavras-chave:
cDNA, mRNA, alinhamento de sequência, similaridade protéicaResumo
O objetivo deste estudo foi buscar in silico os ESTs do Banco de dados do Genoma de Eucalyptus (FORESTs, 2001) correlacionados com genes de alongamento celular envolvidos com crescimento de forma geral. Utilizando bibliotecas de cDNA, nós identificamos agrupamentos de ESTs semelhantes às proteínas controlando alongamento celular registradas no National Center of Biotechnologies Information – NCBI (2002). A busca mostrou similaridade para os seguintes agrupamentos de ESTs: o agrupamento EGEZLV2207D07.g com a enzima esterol delta redutase, EGEQRT4200A01.g com a proteína LKB biossintética de brassinosteróide, EGJMLV2220C06.g com EMBL-transportador mitocondrial half ABC, EGACST6260F07.g e EGMCFB1086B12.g com esterol 22 alfa hidroxilase (CYP90) (P450) e EGEQSL1051D09.g com EGEZSL5201E08.g para a proteína copine.
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