IDENTIFICATION OF POSSIBLE ESTs OF Eucalyptus RELATED TO GENES RESPONSIBLE FOR CELL ELONGATION
DOI:
https://doi.org/10.24278/2178-5031.2012242393Keywords:
cDNA, mRNA, protein similarity, sequence alignmentsAbstract
The objective of study was in silico mining of ESTs from Eucalyptus ESTs database (FORESTs, 2001) correlated with cell elongation genes of growth character. Using cDNA libraries, we identified similar EST clusters to the proteins involved on control of cell elongation have been registered on National Center of Biotechnologies Information – NCBI (2002). The data mining has shown similarities for the following ESTs clusters: EGEZLV2207D07.g with delta sterol reductase, EGEQRT4200A01.g with LKB brassinosteroid biosynthetic protein, EGJMLV2220C06.g with EMBL-transporter of mitochondrial half ABC, EGACST6260F07.g and EGMCFB1086B12.g with steroid 22-α hydroxylase, and EGEQSL1051D09.g with EGEZSL5201E08.g for copine protein.
Downloads
References
ALTSCHUD, S.F. et al. Grapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleics Acids Research, v. 25, p. 3389-3402, 1997.
BENINCASA, M.M.P. Análise de crescimento de plantas (noções básicas). Jaboticabal: FUNEP, 1988. 41 p.
CHENG, L.J. et al. Mutation in nicotianamine aminotransferase stimulated the Fe(II) acquisition system and led to iron accumulation in rice. Plant Physiology, v. 145, p. 1647-1657, 2007.
CHOE, S. et al. Lesions in the sterol delta reductase gene of Arabidopsis cause dwarfism due to a block in brassinosteroid biosynthesis. Plant Journal, v. 21, n. 5, p. 431-443, 2000.
FORESTs: Projeto Genoma do Eucalipto. Disponível em: <https://forests.esalq.usp.br>. Acesso em: 1 set. 2001.
FUJITA, S. et al. Arabidopsis CYP90B1 catalyses theearly C-22 hydroxylation of C27, C28 and C29 sterols. The Plant Journal, v. 45, p. 765-774, 2006.
HUA, J. et al. Plant growth homeostasis is controlled by the Arabidopsis BON1 and BAP1 genes. Genes Dev., v. 15, n. 17, p. 2263-2272, 2001.
KUSHNIR, S. et al. A mutation of the mitochondrial ABC transporter Sta1 leads to dwarfism and chlorosis in the Arabidopsis mutant starik. Plant Cell, v. 13, n. 1, p. 89-100, 2001.
JAGER, C.E. et al. Characterization of two brassinosteroid C-6 oxidase genes in pea1. Plant Physiology, v. 143, p. 1894-1904, 2007.
LECAIN, E. et al. Cloning by metabolic interference in yeast and enzymatic characterization of Arabidopsis thaliana sterol delta 7-reductase. J. Biol. Chem., v. 3, n. 271, p. 10866-73, 1996.
NATIONAL CENTER BIOTECHNOLOGIES INFORMATION – NCBI. Gene Bank. Disponível em: <http://www.ncbi.nlm.nih.gov/>. Acesso em:1 fev. 2002.
NOMURA, T.; BISHOP, G.J. Cytochrome P450s in plant steroid hormone synthesis and metabolism. Phytochem. Rev., v. 5, p. 421-432, 2006.
SCHAFLEITNER, R.; WILHELM, E. Isolation of wound-responsive genes from chestnut (Castanea sativa) microstems by mRNA display and their differential expression upon wounding and infection with the chestnut blight fungus (Chryphonectria parasitica). Physiological and Molecular Plant Pathology, v. 61, n. 6, p. 339-348, 2002.
SCHULTZ, L. et al. Molecular characterization of the brassinosteroid-deficient lkb mutant in pea. Plant Mol. Biol., v. 47, n. 4, p. 491-498, 2001.
SZEKERES, M. et al. Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis. Cell, v. 85, n. 2, p. 171-182, 1996.
TAO, L. et al. Molecular cloning of OSDIM cDNA and its linkage analysis in rice. Rice Genet. Newsl., v. 14, p. 130-132, 1997.
TAKAHASHI, T. et al. The DIMINUTO gene of Arabidopsis is involved in regulating cell elongation. Genes Dev., v. 9, n. 1, p. 97-107, 1995.
TOMSIG, J.L.; SNYDER, S.L.; CREUTZ, C.E.Identification of targets for calcium signaling through the copine family of proteins. J. Biol. Chem., v. 278, n. 12, p. 10048-10054, 2003.
