AMOSTRAGEM PARA ESTIMATIVA DE FREQÜÊNCIAS ALÉLICAS E ÍNDICES DE DIVERSIDADE GENÉTICA EM ESPÉCIES ARBÓREAS

Authors

  • Alexandre Magno Sebbenn Instituto Florestal

DOI:

https://doi.org/10.24278/2178-5031.2002141398

Keywords:

sampling, allele frequencies, indexes of genetic diversity, rare alleles, genetic conservation, genetic drift

Abstract

The aim of this work was to determine the ideal sample size for the estimation of allele frequencies and genetic diversity indexes in forest species, when data of codominant genetic markers are appraised. For so much, it was detennined the sample size when objective to evaluate individuais in a simple population, individuais in several populations, populations of a species, families and progenies/fanulies in a simple population and families and progenies/families in severa! populations. The sample size was estimated based on variances of gene frequencies, incorporating information of the mating system of species, as self-fertilization rate. The ideal sample size to evaluate adults individuais in one or several populations was detemúned to be among 60 to 100 individuais. ln a population structured in families, when alogamus or mixed mating system, predominantly alogamus, species are used, the sampling of 30 families and 10 progenies/family are enough. To autogamus species, the sample size should be of 50 families and 11progemes/families. Finally, to evaluate many populations structured in families it is necessary to adopt the sample of 30 to 10 families/population and 10 progenies/family.

Downloads

Download data is not yet available.

References

AYALA, F. J. ; KIGER, J. A. Modern genetics. Menlo Park: The Benjamin/Cummings Co., 1984. 798 p.

BERG, E. E. ; HAMRlCK, J. L. Quantifícation of diversity at allozyme loci. Canadian Journal Forest Research, Edmonton, v. 27, p. 415-424, 1997.

BROWN, A. H. D. Effícient experimental designs for the estimation of genetic parameters in plant populations. Biometrics, Alexandria, v. 31, p. 145-160, 1975.

____ .; WEIR, S. B. Measuring genetic variability in plant populations. ln: TANKSLEY, S. D.; ORTON, T. J. (Ed.). Isozymes in plant genetics and breeding. Amsterdam: Elsevier Science Publishers, 1983. part A, p. 219-239.

COTTERlLL, P. P.; JAMES, J. W. Ntunber of offspring and plot sizes required for progeny testing. Silvae Genetica, Frankfurt, v. 33, n. 6, p. 203-209, 1984.

COTTERILL, P. P. Short note: number of families and progeny required for provenance testing. Silvae Genetica, Frankfurt, v. 39, n. 2, p. 82-83, 1990.

EL-KASSABY, Y. A. ; SZIKLAI, O. Effect of sample size 011 the precision of the estimate of allozyme frequencies in a natural stand of Douglas-Fir. Egyptian Journal of Genetics and Cytology, Ottawa, v. 24, p. 345-360, 1983.

HAMRlCK, J. L.; LINHART, Y. B.; MITTON, J. B. Relationships between life history characteristic and eletrophoretically detectable genetic variation in plants. Annual Review Ecology and Systematics, Davis, v. 10, p. 173-200, 1979. Rev. lnst. Flor., São Paulo, v. 1 4, n. 1, p. 27-38, jun. 2002.

HAMRICK, J. L.; GODT, M. J. W. Allozyme diversity in plant species. ln: BROWN, A. H. D. et al. (Ed.). Plant population genetics, breeding and genetic resources. Sunderland: Sinauer Press, 1989. p. 43-63.

___ .; SHERMAN-BROYLES, S. L. Factors influencing leveis of genetic diversity in woody plant species. New Forest, Dordrecht, v. 5, p. 95-124, 1992.

NEI, M. Analysis of gene diversity in subdivided populations. Proc. Nat. Acad. Sei., Washington, V. 70, n. 12, p. 3321-3323, 1973. F-statistics and analysis of gene diversity in subdivided populations. Annals of Human Genetics, London, v. 41, p. 225-233, 1977.

RlTLAND, K. ; JAIN, S. A. model for the estimation of outcrossing rate and gene frequencies using independent loci. Heredity, Lund, v. 47, p. 35-52, 1981.

WEIR, B. S. Genetic data analysis II. Methods for discrete population genetic data. Sunderland: North Caroline State University, Sinauer Associates Inc. Pub., 1996. 445 p.

Published

2002-06-07

How to Cite

SEBBENN, A. M. AMOSTRAGEM PARA ESTIMATIVA DE FREQÜÊNCIAS ALÉLICAS E ÍNDICES DE DIVERSIDADE GENÉTICA EM ESPÉCIES ARBÓREAS. Journal of the Forest Institute , São Paulo, v. 14, n. 1, p. 27–38, 2002. DOI: 10.24278/2178-5031.2002141398. Disponível em: https://rif.emnuvens.com.br/revista/article/view/398. Acesso em: 19 sep. 2024.

Issue

Section

Scientific Articles